Oxidative stress due to abnormal induction of reactive oxygen molecules [ROM] is believed to be involved in the etiology of toxicities of many xenobiotics. Evidences suggested that ROM is involved in the nephrotoxicity of widely used synthetic drugs, such as cisplatin. The current investigation was undertaken to explore the protective effect of the ethanolic extract of L. camara flowers, family Verbanaceae, against cisplatin (10 mg/kg; p.o) induced acute renal toxicity in rats characterized by a significant increase in concentrations of serum creatinine, serum urea and blood urea nitrogen (BUN) with change in urine volume, body weight, kidney weight and kidney volume. Effects of the extract (250 and 500 mg/kg; p.o) pretreatments on the renal functions and renal histopathological profile in cisplatin model were evaluated. Cystone (300mg/kg p.o) was used as standard drug. The extract significantly (p < 0.01 and p < 0.001) decreased the levels of serum creatinine, serum urea and BUN and improved morphological parameters and the histoarchitecture revealed improved protection against the acute kidney damage as dose dependent effect with respect to the normal group. DPPH (1,1 Diphenyl-2picryl hydrazyl) free radical scavenging activity. The DPPH radical scavenging activity of L.camara extract carried out and EC50 value was also measured. The nephroprotective effect was due to proanthocyanidins, a flavonoid is known for its high antioxidant property, present in flower ethanolic extract of L. camara.
Keywords: Lantana camara, Cisplatin, Cystone, Antioxidant, Nephroprotective, Histoarchitecture